Composite

Part:BBa_K1983018:Design

Designed by: Vykintas Jauniškis   Group: iGEM16_Vilnius-Lithuania   (2016-10-13)


PheP under constitutive promoter, low strength RBS and terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 98
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers

Primers used for amplification of the fragment:
Uni-FW: GTAGAATTCGCGGCCGCTTCTAG
Uni-RV: GTAGACTGCAGCGGCCGCTACTAG

Primers used for colony PCR screening:

For pSB1C3:
VF2: tgccacctgacgtctaagaa
VR: attaccgcctttgagtgagc

Primers used for cloning a low strength RBS:
RBS-FW: TTGAATTCGCGGCCGCTTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGCTAC
RBS-Low: GCATCTAGAAGTTCCACTCTTTCTCTAGTAGCTAGCATAATACCTAGGACTG

Source

This part is derived from (BBa_B0017). Other parts are derived Escherichia coli and were synthesized by Integrate DNA Technologies.

References